Phytochemical Profile, In Vitro Antimicrobial and Anthelmintic Properties of Dichrostachys Cinerea Root Extracts
DOI:
https://doi.org/10.26437/ajar.v11i2.970Keywords:
Anthelmintic. candida albicans. dichrostachys cinerea. methicillin-resistant. synergistic.Abstract
Purpose: This study sought to assess the active constituents, anthelmintic properties, and antibacterial capabilities of Dichrostachys cinerea.
Design/Methodology/Approach: The research utilises agar well diffusion and microbroth dilution techniques to evaluate the antimicrobial efficacy of D. cinerea's ethanolic root extract. Chemical and GC-MS analyses were employed to identify the phytochemical components. Additionally, an in vitro anthelmintic assessment was performed using Posthuma pheretema.
Findings: Ethanolic extracts of D. cinerea root bark (EDC) were screened against Pheretima posthuma worms, eight (8) bacterial strains, and one (1) fungal strain in vitro. Preliminary phytochemical screening of the extract and GC-MS analysis were also conducted. In this study, the in vitro data demonstrated that EDC exerted both bactericidal and bacteriostatic effects, inhibiting the growth of all tested bacteria and Candida albicans. Combining EDC with Ciprofloxacin inhibited all microbial isolates, including methicillin-resistant Staphylococcus aureus (MRSA).
Research Limitation: This study focused on only one plant part, and all experiments were in vitro, which does not represent the complex in vivo environment. Even though GC-MS identified some compounds, the specific bioactive compounds conferring the bioactivities have not been isolated.
Practical implication: The results of this investigation indicate that the ethanolic root extract of D. cinerea demonstrates potential for development into therapeutic interventions for bacterial, fungal, and helminthic infections.
Social Implication: Data from this research could be utilised to mitigate antimicrobial resistance, thereby improving health outcomes.
Originality/ Value: This study demonstrates, for the first time, the anthelmintic efficacy of the root extract. Furthermore, this research establishes a scientific foundation for subsequent investigations into D. cinerea as a potential source of therapeutic drug candidates.
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